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申请/专利权人：中国热带农业科学院橡胶研究所;中国热带农业科学院三亚研究院

摘要：本发明公开了一种与橡胶树育种性状相关的SNP位点组合及其应用，属于分子生物技术领域。本发明所采用的298个SNP位点与橡胶树的树皮厚度、耐寒性、茎围生产速率、胶乳产量和乳管数量等重要育种性状相关，其可应用于橡胶树的分子标记辅助育种工作中，通过测定橡胶树个体DNA中这298个SNP位点的基因型，快速分析种质资源，确定育种亲本材料中携带的优势等位基因，加快育种进程。此外，298个SNP位点数量相对较少，不仅降低了基因型检测的成本，与全基因组的SNP位点相比，更适用于大量样本的粗筛，且较全基因组的SNP位点而言在对橡胶树育种的综合性状评估上更具优势。

主权项：1.一种与橡胶树育种性状相关的SNP位点组合，其特征在于，包括298个SNP位点，每个SNP位点包含两个不同碱基的等位基因，用于检测该位点的等位基因变化，所述298个SNP位点的物理位置是基于橡胶树野生种质MTVB25A578的基因组序列比对确定的，所述橡胶树野生种质MTVB25A578的基因组序列的版本号为ASM3005281v1，所述298个SNP位点的变异信息如下：所述SNP位点的变异信息采用染色体编号_物理位置_参考基因型等位基因型的形式进行表示：Chr1_15757_GT；Chr1_731023_CT；Chr1_2709764_CT；Chr1_3828026_CG；Chr1_10086984_TC；Chr1_13443998_CA；Chr1_24711557_GA；Chr1_28410342_GA；Chr1_31246482_AG；Chr1_33236009_AT；Chr1_91047897_GA；Chr1_94236268_CT；Chr1_97506370_AG；Chr1_103644378_TA；Chr1_105896364_AC；Chr1_111818657_CA；Chr1_119761444_GA；Chr1_119815929_TA；Chr1_124681987_AG；Chr2_708272_TC；Chr2_1091213_AG；Chr2_1412841_TG；Chr2_6735215_AG；Chr2_8387629_AG；Chr2_8935490_CT；Chr2_14396160_GA；Chr2_17221809_GC；Chr2_23335062_CT；Chr2_52259102_GA；Chr2_65921385_CT；Chr2_71475197_GT；Chr2_77534372_AG；Chr2_85191439_TC；Chr2_102109411_AC；Chr2_107220134_AG；Chr2_112911824_GT；Chr2_116729566_AG；Chr2_116811799_CA；Chr2_123601709_TG；Chr2_124300927_AG；Chr3_1466320_CT；Chr3_1960954_TC；Chr3_4087599_TA；Chr3_5957222_GA；Chr3_12089943_GA；Chr3_12476864_TC；Chr3_14407329_AT；Chr3_19609926_GA；Chr3_25818990_CT；Chr3_26105771_TA；Chr3_28011570_AG；Chr3_55427066_AG；Chr3_65399028_GA；Chr3_78061863_GA；Chr3_83776666_TG；Chr3_87552482_GT；Chr3_87849740_GA；Chr3_89316084_GA；Chr3_95073582_TA；Chr3_97394338_AC；Chr3_97633357_AG；Chr3_99096014_GT；Chr3_105554669_TC；Chr3_108145282_AC；Chr3_110755926_GA；Chr3_111315050_GA；Chr3_113093396_GT；Chr3_115841960_TC；Chr4_4836875_TG；Chr4_5122798_AG；Chr4_13812464_AT；Chr4_14259021_TC；Chr4_20075652_CA；Chr4_20495918_TA；Chr4_27655758_TC；Chr4_28184400_GA；Chr4_101079315_CT；Chr4_104972595_CT；Chr4_109783256_GA；Chr4_110174839_AC；Chr4_111315272_AG；Chr4_115826287_GT；Chr5_5014612_GA；Chr5_5023352_CA；Chr5_5515786_AT；Chr5_9709308_AG；Chr5_12766865_TC；Chr5_15109810_TA；Chr5_22533448_AG；Chr5_25929070_AG；Chr5_65287220_AG；Chr5_73727319_TA；Chr5_88048914_GA；Chr5_99903267_CG；Chr5_105218154_AG；Chr5_108901274_AG；Chr5_110279515_TC；Chr5_111330503_TC；Chr5_111547930_CA；Chr6_810624_TA；Chr6_3036971_AG；Chr6_5034063_TA；Chr6_15910001_CT；Chr6_24457821_GA；Chr6_83782657_CT；Chr6_98816559_GA；Chr6_98989641_TA；Chr6_101179647_TG；Chr6_105804599_GA；Chr6_106597314_GA；Chr6_107794933_TC；Chr7_3136477_AG；Chr7_4403505_CT；Chr7_6790961_CG；Chr7_11042954_GA；Chr7_13203287_TC；Chr7_13586605_TC；Chr7_19002798_CA；Chr7_23498422_TC；Chr7_51574707_GC；Chr7_64866814_TC；Chr7_86545391_GC；Chr7_88082006_AT；Chr7_91885282_TG；Chr7_95451717_GC；Chr7_101803420_TC；Chr8_434205_GT；Chr8_3622765_CT；Chr8_6201463_TC；Chr8_6777431_GT；Chr8_16893876_AT；Chr8_18492509_CA；Chr8_27807713_TC；Chr8_70878100_CT；Chr8_87651866_GT；Chr8_90588981_AC；Chr8_98580941_GA；Chr8_99079868_AG；Chr8_100597325_CG；Chr9_1813979_CG；Chr9_5709469_CA；Chr9_7401382_CA；Chr9_7664635_AC；Chr9_14855094_TC；Chr9_17665225_TC；Chr9_18653960_CT；Chr9_20264134_AT；Chr9_20707073_AG；Chr9_24502054_AC；Chr9_24854055_AC；Chr9_34308910_CT；Chr9_34873585_TC；Chr9_75435371_CT；Chr9_77610735_GA；Chr9_86585759_TA；Chr9_88315788_AG；Chr9_91502195_GA；Chr9_95436365_TA；Chr9_97867065_GA；Chr10_1470878_AT；Chr10_5824840_GC；Chr10_8136996_AT；Chr10_10507992_GA；Chr10_15719936_TA；Chr10_27591981_CA；Chr10_87585142_CG；Chr10_90419402_CA；Chr10_90421003_GA；Chr10_94722518_GA；Chr10_96108456_CT；Chr10_98147463_CG；Chr11_678127_CT；Chr11_736365_AG；Chr11_1115977_CG；Chr11_7196081_GA；Chr11_10848831_GC；Chr11_14918947_TC；Chr11_18839922_CG；Chr11_22924094_CT；Chr11_52683514_TG；Chr11_72064153_AG；Chr11_73664189_CT；Chr11_74555756_AG；Chr11_75121758_CT；Chr11_82668555_TG；Chr11_91501311_TG；Chr11_93188716_TG；Chr11_95071313_CT；Chr11_96208291_CT；Chr11_98880506_CT；Chr12_684080_CA；Chr12_2931612_TC；Chr12_6899137_TA；Chr12_7707046_AG；Chr12_12156787_AT；Chr12_13341210_TA；Chr12_16307269_CT；Chr12_16328314_AG；Chr12_22494251_TC；Chr12_23601414_GC；Chr12_26680799_CG；Chr12_26973865_CA；Chr12_32544120_AT；Chr12_35993093_TC；Chr12_37011239_CA；Chr12_38908419_AC；Chr12_40517500_AG；Chr12_45207311_TC；Chr12_45440599_TC；Chr12_69549182_AG；Chr12_79376136_CG；Chr13_4356832_GT；Chr13_8875751_TC；Chr13_13255560_GT；Chr13_13918244_AG；Chr13_15886975_TA；Chr13_16924693_AG；Chr13_25851280_TC；Chr13_31683642_AG；Chr13_48641671_CG；Chr13_69350364_GA；Chr13_69392735_GA；Chr13_70291223_TA；Chr13_77454106_GA；Chr13_77704508_TA；Chr13_78200581_GA；Chr13_83025236_GA；Chr13_83094380_TG；Chr13_86155184_CT；Chr13_89160461_GA；Chr13_89846004_TG；Chr14_3754938_GA；Chr14_4002023_AG；Chr14_5279683_TG；Chr14_5988595_CT；Chr14_16631275_GT；Chr14_16668074_CT；Chr14_23975735_CT；Chr14_24930605_TA；Chr14_29279540_CT；Chr14_68126151_AG；Chr14_68309126_GA；Chr14_69200024_AG；Chr14_74583350_AC；Chr14_81298104_CA；Chr14_83285431_CG；Chr14_89010870_GA；Chr14_91155262_GT；Chr15_2986762_CG；Chr15_3196798_CA；Chr15_8687335_GA；Chr15_23754687_AG；Chr15_35180027_AG；Chr15_40670852_CT；Chr15_47894762_GT；Chr15_52881987_TA；Chr15_53642786_GT；Chr15_54447431_TC；Chr15_63208884_CT；Chr15_66357157_GT；Chr15_70990505_AG；Chr15_73033483_TC；Chr16_8093869_TC；Chr16_8130467_GA；Chr16_9976916_GA；Chr16_41093450_CA；Chr16_41892017_TC；Chr16_43339238_AC；Chr16_47328276_TC；Chr16_50459414_TC；Chr16_55859604_AG；Chr16_61536320_AG；Chr16_64893652_AC；Chr16_69033394_CT；Chr16_70631013_CA；Chr17_4332955_GC；Chr17_5300940_CT；Chr17_11344297_AG；Chr17_14450909_CT；Chr17_24540321_GA；Chr17_25427335_GA；Chr17_39194976_GA；Chr17_47016224_AG；Chr17_51749822_AG；Chr17_55881919_CT；Chr17_58004068_AG；Chr18_2465717_TA；Chr18_7859243_AG；Chr18_28775131_CT；Chr18_29989533_GA；Chr18_35440358_CT；Chr18_41190847_AG；Chr18_42439396_CA；Chr18_42574751_AC；Chr18_45891002_CT；Chr18_46892894_CT；Chr18_50817494_GC；Chr18_51611167_TC；所述育种性状包括树皮厚度、耐寒性、茎围生产速率、胶乳产量和乳管数量；与树皮厚度相关的52个SNP位点及其优势等位基因如下：Chr1_731023_T；Chr1_10086984_T；Chr1_28410342_A；Chr1_91047897_G；Chr1_103644378_A；Chr1_119761444_A；Chr2_708272_T；Chr2_107220134_G；Chr2_116811799_A；Chr3_12476864_C；Chr3_28011570_A；Chr3_65399028_G；Chr3_89316084_G；Chr3_97394338_A；Chr3_110755926_G；Chr4_5122798_A；Chr4_104972595_T；Chr4_115826287_T；Chr5_5023352_C；Chr5_12766865_C；Chr5_108901274_A；Chr6_810624_T；Chr6_101179647_T；Chr7_11042954_A；Chr7_91885282_T；Chr8_6777431_G；Chr8_87651866_G；Chr9_5709469_C；Chr9_14855094_T；Chr9_20707073_A；Chr9_34873585_T；Chr9_97867065_G；Chr10_1470878_A；Chr10_87585142_C；Chr11_14918947_T；Chr11_73664189_C；Chr11_93188716_T；Chr12_2931612_C；Chr12_22494251_T；Chr13_70291223_T；Chr13_83025236_G；Chr14_89010870_G；Chr15_66357157_G；Chr15_73033483_T；Chr16_8093869_T；Chr16_41892017_T；Chr16_50459414_T；Chr16_64893652_A；Chr17_11344297_G；Chr17_47016224_A；Chr17_55881919_C；Chr18_45891002_T；与耐寒性相关的39个SNP位点及其优势等位基因如下：Chr1_15757_G；Chr2_8387629_A；Chr2_112911824_T；Chr2_123601709_T；Chr3_1466320_T；Chr3_105554669_C；Chr3_111315050_A；Chr4_111315272_G；Chr5_5515786_A；Chr6_98816559_A；Chr7_4403505_C；Chr8_18492509_C；Chr8_27807713_T；Chr8_100597325_C；Chr9_7664635_C；Chr9_17665225_T；Chr9_24854055_A；Chr9_34308910_T；Chr10_15719936_T；Chr10_94722518_A；Chr11_18839922_C；Chr11_75121758_T；Chr11_96208291_C；Chr12_7707046_G；Chr12_16307269_C；Chr12_26973865_A；Chr12_40517500_A；Chr13_16924693_G；Chr13_31683642_A；Chr13_86155184_T；Chr14_5988595_C；Chr14_16668074_C；Chr14_24930605_A；Chr14_81298104_A；Chr16_41093450_A；Chr17_24540321_A；Chr18_2465717_A；Chr18_29989533_A；Chr18_46892894_T；与茎围生产速率相关的61个SNP位点及其优势等位基因如下：Chr1_2709764_C；Chr1_94236268_C；Chr1_111818657_C；Chr2_6735215_A；Chr2_17221809_C；Chr2_116729566_A；Chr3_5957222_A；Chr3_19609926_A；Chr3_25818990_T；Chr3_87849740_A；Chr3_95073582_A；Chr3_115841960_T；Chr4_14259021_C；Chr4_28184400_G；Chr5_15109810_A；Chr5_25929070_G；Chr5_105218154_G；Chr5_111547930_A；Chr6_106597314_A；Chr7_13203287_C；Chr7_95451717_G；Chr9_1813979_C；Chr9_7401382_C；Chr9_20264134_T；Chr9_75435371_T；Chr9_88315788_A；Chr10_8136996_A；Chr10_90421003_G；Chr10_96108456_T；Chr11_736365_G；Chr11_10848831_C；Chr11_91501311_G；Chr12_16328314_G；Chr12_26680799_G；Chr12_35993093_T；Chr12_45207311_C；Chr12_69549182_G；Chr12_79376136_G；Chr13_4356832_G；Chr13_15886975_T；Chr13_25851280_C；Chr13_48641671_C；Chr13_77704508_A；Chr13_89160461_A；Chr14_3754938_G；Chr14_16631275_G；Chr14_29279540_T；Chr14_68309126_G；Chr14_91155262_T；Chr15_3196798_A；Chr15_47894762_T；Chr15_53642786_G；Chr15_63208884_T；Chr16_43339238_A；Chr16_61536320_G；Chr17_5300940_T；Chr17_14450909_T；Chr17_51749822_A；Chr18_28775131_T；Chr18_41190847_A；Chr18_51611167_T；与胶乳产量相关的82个SNP位点及其优势等位基因如下：Chr1_731023_T；Chr1_13443998_A；Chr1_24711557_A；Chr1_31246482_A；Chr1_105896364_C；Chr1_124681987_A；Chr2_1412841_T；Chr2_23335062_C；Chr2_52259102_G；Chr2_65921385_C；Chr2_71475197_G；Chr2_85191439_T；Chr2_124300927_A；Chr3_4087599_T；Chr3_14407329_A；Chr3_26105771_T；Chr3_55427066_A；Chr3_78061863_G；Chr3_87552482_G；Chr3_99096014_G；Chr3_108145282_A；Chr4_4836875_T；Chr4_20495918_T；Chr4_101079315_C；Chr4_110174839_A；Chr5_9709308_A；Chr5_22533448_A；Chr5_65287220_A；Chr5_73727319_T；Chr5_88048914_G；Chr5_110279515_T；Chr6_3036971_A；Chr6_15910001_C；Chr6_24457821_A；Chr6_83782657_C；Chr6_98989641_T；Chr6_107794933_T；Chr7_6790961_C；Chr7_13586605_C；Chr7_23498422_T；Chr7_86545391_G；Chr8_3622765_C；Chr8_16893876_A；Chr8_70878100_T；Chr8_90588981_A；Chr8_98580941_G；Chr9_24502054_A；Chr9_77610735_G；Chr9_86585759_T；Chr9_95436365_A；Chr10_10507992_G；Chr10_98147463_C；Chr11_1115977_C；Chr11_7196081_G；Chr11_22924094_T；Chr11_72064153_A；Chr11_98880506_C；Chr12_684080_C；Chr12_12156787_T；Chr12_37011239_C；Chr13_8875751_C；Chr13_13918244_G；Chr13_69392735_G；Chr13_77454106_G；Chr13_83094380_T；Chr13_89846004_T；Chr14_5279683_T；Chr14_23975735_C；Chr14_68126151_G；Chr14_74583350_A；Chr15_8687335_G；Chr15_23754687_A；Chr15_40670852_C；Chr15_52881987_T；Chr16_8130467_A；Chr16_55859604_A；Chr16_69033394_C；Chr17_25427335_G；Chr17_39194976_A；Chr17_58004068_A；Chr18_7859243_A；Chr18_42574751_A；与乳管数量相关的65个SNP位点及其优势等位基因如下：Chr1_3828026_G；Chr1_33236009_T；Chr1_97506370_A；Chr1_119815929_T；Chr2_1091213_A；Chr2_8935490_T；Chr2_14396160_G；Chr2_77534372_A；Chr2_102109411_A；Chr3_1960954_T；Chr3_12089943_A；Chr3_83776666_G；Chr3_97633357_G；Chr3_113093396_G；Chr4_13812464_A；Chr4_20075652_A；Chr4_27655758_T；Chr4_109783256_A；Chr5_5014612_G；Chr5_99903267_G；Chr5_111330503_T；Chr6_5034063_T；Chr6_105804599_A；Chr7_3136477_G；Chr7_19002798_A；Chr7_51574707_G；Chr7_64866814_T；Chr7_88082006_A；Chr7_101803420_C；Chr8_434205_T；Chr8_6201463_T；Chr8_99079868_G；Chr9_18653960_C；Chr9_91502195_G；Chr10_5824840_C；Chr10_27591981_A；Chr10_90419402_A；Chr11_678127_C；Chr11_52683514_T；Chr11_74555756_A；Chr11_82668555_T；Chr11_95071313_T；Chr12_6899137_T；Chr12_13341210_A；Chr12_23601414_G；Chr12_32544120_A；Chr12_38908419_C；Chr12_45440599_C；Chr13_13255560_G；Chr13_69350364_G；Chr13_78200581_G；Chr14_4002023_A；Chr14_69200024_G；Chr14_83285431_G；Chr15_2986762_G；Chr15_35180027_A；Chr15_54447431_T；Chr15_70990505_G；Chr16_9976916_A；Chr16_47328276_T；Chr16_70631013_A；Chr17_4332955_G；Chr18_35440358_C；Chr18_42439396_A；Chr18_50817494_C。

全文数据：

权利要求：

百度查询： 中国热带农业科学院橡胶研究所 中国热带农业科学院三亚研究院 一种与橡胶树育种性状相关的SNP位点组合及其应用