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申请/专利权人：江苏省农业科学院

摘要：本发明公开了一种元宝枫的改良培养基及繁殖方法，属于植物组织培养繁殖技术领域，解决了现有技术中采用嫁接和扦插的方式繁殖元宝枫成本高、采用播种的方式繁殖元宝枫个体间存在很大差异导致不利于大规模工厂化生产的问题。该培养基包括NH4NO3600～800mgL、KNO3800～1000mgL、MgSO4100～300mgL、KH2PO41～100mgL、CaCl2·2H2O100～300mgL、Na2·EDTA·2H2O10～50mgL、FeSO4·7H2O10～50mgL、H3BO31～10mgL、Na2MoO4·2H2O0.01～1mgL等。该方法包括将外植体依次接种于外植体萌发培养基、丛生芽诱导培养基和生根培养基上，生根成苗后移栽。该培养基及繁殖方法可用于元宝枫微繁殖。

主权项：1.一种元宝枫的繁殖方法，其特征在于，包括如下步骤：步骤1：采集元宝枫母树当年生枝条，切割成带芽茎段，作为外植体；步骤2：对外植体进行消毒处理；步骤3：去掉外植体的腋叶和基部的切口，将外植体接种于外植体萌发培养基上，使得芽体展叶，获得无菌苗；步骤4：将无菌苗的新生茎段置于丛生芽诱导培养基上，获得丛生芽；步骤5：将丛生芽置于生根培养基，生根成苗后移栽，完成元宝枫的繁殖；所述外植体萌发培养基的组成按质量体积浓度计为：NH4NO3650mgL、KNO3850mgL、MgSO4200mgL、KH2PO4100mgL、CaCl2·2H2O300mgL、Na2·EDTA·2H2O30mgL、FeSO4·7H2O30mgL、H3BO310mgL、Na2MoO4·2H2O0.05mgL、CuSO4·5H2O0.5mgL、MnSO4·4H2O20mgL、ZnSO4·7H2O50mgL、生物素H0.01mgL、烟酸20mgL、盐酸硫胺素10mgL、盐酸吡哆醇0.1mgL、肌醇1mgL、甘氨酸5mgL、叶酸5mgL、IBA0.1mgL、蔗糖20.0gL和琼脂6.0gL，pH为5.8；所述丛生芽诱导培养基的组成按质量体积浓度计为：NH4NO3650mgL、KNO3850mgL、MgSO4200mgL、KH2PO4100mgL、CaCl2·2H2O300mgL、Na2·EDTA·2H2O30mgL、FeSO4·7H2O30mgL、H3BO310mgL、Na2MoO4·2H2O0.05mgL、CuSO4·5H2O0.5mgL、MnSO4·4H2O20mgL、ZnSO4·7H2O50mgL、生物素H0.01mgL、烟酸20mgL、盐酸硫胺素10mgL、盐酸吡哆醇0.1mgL、肌醇1mgL、甘氨酸5mgL、叶酸5mgL、KT0.2mgL、IBA0.2mgL、蔗糖20.0gL和琼脂6.0gL，pH为5.8；所述生根培养基的组成按质量体积浓度计为：NH4NO3650mgL、KNO3850mgL、MgSO4200mgL、KH2PO4100mgL、CaCl2·2H2O300mgL、Na2·EDTA·2H2O30mgL、FeSO4·7H2O30mgL、H3BO310mgL、Na2MoO4·2H2O0.05mgL、CuSO4·5H2O0.5mgL、MnSO4·4H2O20mgL、ZnSO4·7H2O50mgL、生物素H0.01mgL、烟酸20mgL、盐酸硫胺素10mgL、盐酸吡哆醇0.1mgL、肌醇1mgL、甘氨酸5mgL和叶酸5mgL、IBA0.2mgL、蔗糖20.0gL和琼脂6.0gL，pH为5.8；采用所述繁殖方法对元宝枫进行微繁殖，繁殖系数为5.10±0.84；或者，所述外植体萌发培养基的组成按质量体积浓度计为：NH4NO3690mgL、KNO3950mgL、MgSO4280mgL、KH2PO450mgL、CaCl2·2H2O200mgL、Na2·EDTA·2H2O45mgL、FeSO4·7H2O45mgL、H3BO35mgL、Na2MoO4·2H2O1mgL、CuSO4·5H2O0.2mgL、MnSO4·4H2O45mgL、ZnSO4·7H2O20mgL、生物素H0.4mgL、烟酸6mgL、盐酸硫胺素1mgL、盐酸吡哆醇8mgL、肌醇45mgL、甘氨酸1mgL、叶酸1mgL、IBA0.2mgL、蔗糖15.0gL和琼脂6.0gL，pH为5.8；所述丛生芽诱导培养基的组成按质量体积浓度计为：NH4NO3690mgL、KNO3950mgL、MgSO4280mgL、KH2PO450mgL、CaCl2·2H2O200mgL、Na2·EDTA·2H2O45mgL、FeSO4·7H2O45mgL、H3BO35mgL、Na2MoO4·2H2O1mgL、CuSO4·5H2O0.2mgL、MnSO4·4H2O45mgL、ZnSO4·7H2O20mgL、生物素H0.4mgL、烟酸6mgL、盐酸硫胺素1mgL、盐酸吡哆醇8mgL、肌醇45mgL、甘氨酸1mgL、叶酸1mgL、KT0.4mgL、IBA0.1mgL、蔗糖15.0gL和琼脂5.6gL，pH为5.6；所述生根培养基的组成按质量体积浓度计为：NH4NO3690mgL、KNO3950mgL、MgSO4280mgL、KH2PO450mgL、CaCl2·2H2O200mgL、Na2·EDTA·2H2O45mgL、FeSO4·7H2O45mgL、H3BO35mgL、Na2MoO4·2H2O1mgL、CuSO4·5H2O0.2mgL、MnSO4·4H2O45mgL、ZnSO4·7H2O20mgL、生物素H0.4mgL、烟酸6mgL、盐酸硫胺素1mgL、盐酸吡哆醇8mgL、肌醇45mgL、甘氨酸1mgL、叶酸1mgL、IBA0.4mgL、蔗糖15.0gL和琼脂5.6gL，pH为5.6；采用所述繁殖方法对元宝枫进行微繁殖，繁殖系数为3.27±0.93；或者，所述外植体萌发培养基的组成按质量体积浓度计为：NH4NO3800mgL、KNO3800mgL、MgSO4120mgL、KH2PO410mgL、CaCl2·2H2O120mgL、Na2·EDTA·2H2O15mgL、FeSO4·7H2O15mgL、H3BO32mgL、Na2MoO4·2H2O0.02mgL、CuSO4·5H2O0.1mgL、MnSO4·4H2O10mgL、ZnSO4·7H2O5mgL、生物素H0.005mgL、烟酸1mgL、盐酸硫胺素0.1mgL、盐酸吡哆醇7mgL、肌醇20mgL、甘氨酸8mgL、叶酸8mgL、IBA0.2mgL、蔗糖15.0gL和琼脂5.6gL，pH为5.6；所述丛生芽诱导培养基的组成按质量体积浓度计为：NH4NO3800mgL、KNO3800mgL、MgSO4120mgL、KH2PO410mgL、CaCl2·2H2O120mgL、Na2·EDTA·2H2O15mgL、FeSO4·7H2O15mgL、H3BO32mgL、Na2MoO4·2H2O0.02mgL、CuSO4·5H2O0.1mgL、MnSO4·4H2O10mgL、ZnSO4·7H2O5mgL、生物素H0.005mgL、烟酸1mgL、盐酸硫胺素0.1mgL、盐酸吡哆醇7mgL、肌醇20mgL、甘氨酸8mgL、叶酸8mgL、KT0.4mgL、IBA0.1mgL、蔗糖15.0gL和琼脂5.6gL，pH为5.6；所述生根培养基的组成按质量体积浓度计为：NH4NO3800mgL、KNO3800mgL、MgSO4120mgL、KH2PO410mgL、CaCl2·2H2O120mgL、Na2·EDTA·2H2O15mgL、FeSO4·7H2O15mgL、H3BO32mgL、Na2MoO4·2H2O0.02mgL、CuSO4·5H2O0.1mgL、MnSO4·4H2O10mgL、ZnSO4·7H2O5mgL、生物素H0.005mgL、烟酸1mgL、盐酸硫胺素0.1mgL、盐酸吡哆醇7mgL、肌醇20mgL、甘氨酸8mgL、叶酸8mgL、IBA0.4mgL、蔗糖15.0gL和琼脂5.6gL，pH为5.6；采用所述繁殖方法对元宝枫进行微繁殖，繁殖系数为2.13±0.79；生根率达95%以上。

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